Celiac Disease

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April 19th, 2016

The Gluten ELISA Test Kit

By Kanaaz Pereira

If you have celiac disease, you may rely on commercial test kits for detecting gluten in food. But how reliable are these test kits for gluten detection? Dr. Joseph Murray, M.D., evaluates the ELISA test kits, based upon a study published in Cereal Chemistry.

The team of researchers who set out to evaluate the accuracy of 14 ELISA kits for gluten detection found that none of the currently available ELISA methods can accurately detect and quantify gluten in all cases. In the face of these results, Dr. Murray has the following recommendations for individuals who use the kits:

  • The FDA allows food products containing less than 20 parts per million of gluten to be labeled as gluten-free.
  • Be very cautious, and repeat the test multiple times.
  • Pay attention to the quality of the test.
  • Make sure that the test is appropriate for the matrix of the food type.

The authors of this study conclude that further improvements are urgently needed, and recommend focusing on competitive formats, improving extraction methods, and the detection of relevant gluten peptides.

Read the full study online here.

For more information about celiac disease, visit mayoclinic.org/celiac disease.

Dr. Murray is a gastroenterologist and celiac disease expert at Mayo Clinic.

 

 

Tags: celiac disease, Cereal Chemistry, ELISA test, FDA, gluten, gluten free, Joseph Murray, Study Findings, Tips from Clinicians

Comment


AndyF
@andyf

Posts: 1
Joined: Jun 30, 2016
Posted by @andyf, Jun 29, 2016

The unreliability of the tests is a little disheartening for people with coeliac disease. Unfortunately there are few among us who would be prepared to repeat the test multiple times, this would result in a lot of expense if it were to be done regularly. Further challenges exist in Australia where the “gluten free” labeling limit is 3ppm – https://www.kooeesnacks.com.au/gluten-free-mean/

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